SARS-CoV-2 RdRp Gene Detection Using an Electrochemiluminescent Sensor Based on MXene and Integrating 3D-DNA Walker and CRISPR-Cas12a Trans-cleavage Activity

According to the WHO, halting the spread of the pandemic and averting the appearance of new symptoms depend on the timely detection and treatment of SARS-CoV-2 infection (Severe Acute Respiratory Syndrome Coronavirus 2). A rapid diagnosis and treatment of SARS-CoV-2 infection is needed as epidemic monitoring is intensified. Using a 3D DNA walker, it may be necessary to amplify double-stranded DNA in order to activate the CRISPR-Cas12a system. SARS-RdRp CoV-2's gene might be detected in humans using electro chemiluminescent (ECL) biosensors thanks to this technique. CRISPR-cleavage Cas12a was triggered when double-stranded DNA was created, according to the researchers. A biosensor based on MXene, PEI-Ru@Ti3C2@AuNPs, and ECL materials of our own design and invention were used to identify this gene. When the CRISPR-Cas12a gene is active, DNA linked to the sensor surface that is single-stranded moves away from the electrode surface. Signals from sensors rise as a result. Analyzing the quantity of this gene in the blood using electrochemiluminescence may be possible in the future. Researchers that employed this approach to identify SARS-CoV-2 RdRp reported a detection limit of 12.8 aM. New composite materials and the use of CRISPR-Cas12a have made it possible to identify SARS-CoV-2 nucleic acids in clinical situations. To explore whether CRISPR-Cas12a-based biosensors can detect ECL, they are now being evaluated in clinical settings.

Keywords: SARS-CoV-2, Gene Detection, Electrochemiluminescent, Trans-cleavage Activity

According to the WHO, halting the spread of the pandemic and averting the appearance of new symptoms depend on the timely detection and treatment of SARS-CoV-2 infection (Severe Acute Respiratory Syndrome Coronavirus 2). A rapid diagnosis and treatment of SARS-CoV-2 infection is needed as epidemic monitoring is intensified. Using a 3D DNA walker, it may be necessary to amplify double-stranded DNA in order to activate the CRISPR-Cas12a system. SARS-RdRp CoV-2's gene might be detected in humans using electro chemiluminescent (ECL) biosensors thanks to this technique. CRISPR-cleavage Cas12a was triggered when double-stranded DNA was created, according to the researchers. A biosensor based on MXene, PEI-Ru@Ti3C2@AuNPs, and ECL materials of our own design and invention were used to identify this gene. When the CRISPR-Cas12a gene is active, DNA linked to the sensor surface that is single-stranded moves away from the electrode surface. Signals from sensors rise as a result. Analyzing the quantity of this gene in the blood using electrochemiluminescence may be possible in the future. Researchers that employed this approach to identify SARS-CoV-2 RdRp reported a detection limit of 12.8 aM. New composite materials and the use of CRISPR-Cas12a have made it possible to identify SARS-CoV-2 nucleic acids in clinical situations. To explore whether CRISPR-Cas12a-based biosensors can detect ECL, they are now being evaluated in clinical settings.

Anahtar Kelimeler: SARS-CoV-2, Gene Detection, Electrochemiluminescent, Trans-cleavage Activity

A-Kadhim, M. M-H., Abdul-Hussin, I. F. & Al-Khatee, D. S. M. (2022). SARS-CoV-2 RdRp Gene Detection Using an Electrochemiluminescent Sensor Based on MXene and Integrating 3D-DNA Walker and CRISPR-Cas12a Trans-cleavage Activity. Journal of Current Researches on Health Sector, 12 (2), 111-130.