Quantification Analysis of Betahistine in Tablet Dosage Forms Using HPLC-ZIC-HILIC with Ultraviolet Detection
Ayad Kadhim FADHİLA simple ZIC-HILIC-UV technique was developed and applied to determine Betahistine (N-methyl-2-(pyridin-2-yl) ethanamine) in tablet dosage forms. The separation took place in the ZIC1 and ZIC5 homemade stationary phases. Mix an acetate buffer and acetonitrile in the mobile gradient phase and detect at 235 nm. The Betahistine conduct with varying acetate buffer concentration, buffer pH, and acetonitrile has been tested. The results have established the mechanism of separation is based on the hydrophilic partitioning of Betahistine. For the ZIC1 and ZIC4 stationary phases, the developed ZIC-HILIC techniques had high precision (0.99%), linear ranges of 0.01-10 ppm, and detection limits of 0.0074 and 0.0060 ppm, with a coefficient of determination of 0.9999 and 0.9998, respectively. The method was a valuable alternative to Betahistine quantification in tablet dosage forms.
Keywords: Vertigo, Anti-inflammatory drugs, ZIC-HILIC, Betahistine, Tablet dosage forms
Quantification Analysis of Betahistine in Tablet Dosage Forms Using HPLC-ZIC-HILIC with Ultraviolet Detection
A simple ZIC-HILIC-UV technique was developed and applied to determine Betahistine (N-methyl-2-(pyridin-2-yl) ethanamine) in tablet dosage forms. The separation took place in the ZIC1 and ZIC5 homemade stationary phases. Mix an acetate buffer and acetonitrile in the mobile gradient phase and detect at 235 nm. The Betahistine conduct with varying acetate buffer concentration, buffer pH, and acetonitrile has been tested. The results have established the mechanism of separation is based on the hydrophilic partitioning of Betahistine. For the ZIC1 and ZIC4 stationary phases, the developed ZIC-HILIC techniques had high precision (0.99%), linear ranges of 0.01-10 ppm, and detection limits of 0.0074 and 0.0060 ppm, with a coefficient of determination of 0.9999 and 0.9998, respectively. The method was a valuable alternative to Betahistine quantification in tablet dosage forms.
Anahtar Kelimeler: Vertigo, Anti-inflammatory drugs, ZIC-HILIC, Betahistine, Tablet dosage forms
For Cited
Fadhil, A. K. (2022). Quantification Analysis of Betahistine in Tablet Dosage Forms Using HPLC-ZIC-HILIC with Ultraviolet Detection. Journal of Current Researches on Educational Studies, 12 (1), 177-186.